Device

Part:BBa_K3024009

Designed by: Tobias Willi   Group: iGEM19_Stockholm   (2019-09-28)


Inducible C-protein construct - Regulate lysogenic module

This biobrick expresses C protein from P2 bacteriophage, a transcription factor that -together with Cox transcription factor- regulates the switch between lysogenic/lytic life cycles. C protein activates genes that promote lysogeny. Since C gene is expressed under pBAd promoter in this construct, expression of C protein is dependent on arabinose concentration. Furthermore, this part is repressible by TetR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1092
    Illegal AgeI site found at 1204
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None